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bglii  (New England Biolabs)


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    Structured Review

    New England Biolabs bglii
    Bglii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 2840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bglii/BglII/pmc13122177-108-31-32
    Average 98 stars, based on 2840 article reviews
    bglii - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Amplification:

    Article Title: Association of IL6 rs1800795, TNF rs1800629, CCL2 rs1024611 and VEGFA rs699947 Polymorphisms with Bladder Cancer Risk, Tumor Aggressiveness, and HRV Parameters of Autonomic Nervous System Regulation
    Article Snippet: .. The amplified 325 bp PCR product was digested with BglII (New England Biolabs, Ipswich, Massachusetts, USA). ..

    Polymerase Chain Reaction:

    Article Title: Association of IL6 rs1800795, TNF rs1800629, CCL2 rs1024611 and VEGFA rs699947 Polymorphisms with Bladder Cancer Risk, Tumor Aggressiveness, and HRV Parameters of Autonomic Nervous System Regulation
    Article Snippet: .. The amplified 325 bp PCR product was digested with BglII (New England Biolabs, Ipswich, Massachusetts, USA). ..

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein
    Article Snippet: Site mutations in EGFP (#1–#4, and DEVAG) were generated using a gene synthesis service (Bioneer, Daejeon, South Korea). .. The PCR products of mutant EGFP genes (#1 ∼#4) and EGFP were cloned into the pCAGGS vector linearized with BglII (Cat. #R0144L, NEB, Ipswich, MA, USA) using the In-Fusion HD Cloning Kit (#102518, Takara Bio, Shiga, Japan). .. To integrate EGFP #4 in the safe-harbor locus, such as human AAVS1 (hAAVS1) and porcine ROSA26 (pROSA26), the PCR product of EGFP #4 was cloned into the BglII restriction enzyme site of AAVS1 SA-2A-puro-pA donor vector (#22075, Addgene, Watertown, MA, USA) using the In-Fusion HD Cloning Kit (TaKaRa Bio).

    Plasmid Preparation:

    Article Title: Competition for the conserved branch point sequence influences physiological outcomes in pre-mRNA splicing
    Article Snippet: .. These and the pDUP51 plasmid were digested with ApaI and BglII (NEB), then ligated into pDUP51 at the ApaI and BglII restriction sites. ..

    Construct:

    Article Title: Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genome.
    Article Snippet: .. For the full-length and constructs ( 1 and 2) in pUAST, KpnI-HF (NEB, #R3142S) and NotI (NEB, #R0189S) were employed; UP (upstream region) was achieved using NdeI (NEB, #R0111S) and BglII (NEB, #R0144S); TATA promoter using SbfI-HF (NEB, #R3642S) and BglII. .. For insertion of RMND5A and ANAPC1 sequences into the pGL3 vector, digestion was performed using KpnI and HindIII (NEB, #R0104S).

    Article Title: Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genome
    Article Snippet: .. For the full-length and Δ constructs (Δ1 and Δ2) in pUAST, KpnI-HF (NEB, #R3142S) and NotI (NEB, #R0189S) were employed; Δ UP (upstream region) was achieved using NdeI (NEB, #R0111S) and BglII (NEB, #R0144S); Δ TATA promoter using SbfI-HF (NEB, #R3642S) and BglII. .. For insertion of RMND5A and ANAPC1 sequences into the pGL3 vector, digestion was performed using KpnI and HindIII (NEB, #R0104S).

    Mutagenesis:

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein
    Article Snippet: Site mutations in EGFP (#1–#4, and DEVAG) were generated using a gene synthesis service (Bioneer, Daejeon, South Korea). .. The PCR products of mutant EGFP genes (#1 ∼#4) and EGFP were cloned into the pCAGGS vector linearized with BglII (Cat. #R0144L, NEB, Ipswich, MA, USA) using the In-Fusion HD Cloning Kit (#102518, Takara Bio, Shiga, Japan). .. To integrate EGFP #4 in the safe-harbor locus, such as human AAVS1 (hAAVS1) and porcine ROSA26 (pROSA26), the PCR product of EGFP #4 was cloned into the BglII restriction enzyme site of AAVS1 SA-2A-puro-pA donor vector (#22075, Addgene, Watertown, MA, USA) using the In-Fusion HD Cloning Kit (TaKaRa Bio).

    Clone Assay:

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein
    Article Snippet: Site mutations in EGFP (#1–#4, and DEVAG) were generated using a gene synthesis service (Bioneer, Daejeon, South Korea). .. The PCR products of mutant EGFP genes (#1 ∼#4) and EGFP were cloned into the pCAGGS vector linearized with BglII (Cat. #R0144L, NEB, Ipswich, MA, USA) using the In-Fusion HD Cloning Kit (#102518, Takara Bio, Shiga, Japan). .. To integrate EGFP #4 in the safe-harbor locus, such as human AAVS1 (hAAVS1) and porcine ROSA26 (pROSA26), the PCR product of EGFP #4 was cloned into the BglII restriction enzyme site of AAVS1 SA-2A-puro-pA donor vector (#22075, Addgene, Watertown, MA, USA) using the In-Fusion HD Cloning Kit (TaKaRa Bio).

    Cloning:

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein
    Article Snippet: Site mutations in EGFP (#1–#4, and DEVAG) were generated using a gene synthesis service (Bioneer, Daejeon, South Korea). .. The PCR products of mutant EGFP genes (#1 ∼#4) and EGFP were cloned into the pCAGGS vector linearized with BglII (Cat. #R0144L, NEB, Ipswich, MA, USA) using the In-Fusion HD Cloning Kit (#102518, Takara Bio, Shiga, Japan). .. To integrate EGFP #4 in the safe-harbor locus, such as human AAVS1 (hAAVS1) and porcine ROSA26 (pROSA26), the PCR product of EGFP #4 was cloned into the BglII restriction enzyme site of AAVS1 SA-2A-puro-pA donor vector (#22075, Addgene, Watertown, MA, USA) using the In-Fusion HD Cloning Kit (TaKaRa Bio).



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    Image Search Results


    Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between RFC10 prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.

    Journal: STAR Protocols

    Article Title: Protocol for condensate-based stabilization of gene circuit dynamics under growth-mediated dilution in E. coli

    doi: 10.1016/j.xpro.2026.104536

    Figure Lengend Snippet: Design of gene circuits used in this protocol (A) DNA sequence of B0034-MCS1-pSB1A2 between RFC10 prefix and suffix. The solid green arrow marks the start codon of the open reading frame. (B) Schematic diagrams of synthetic gene circuits constructed and characterized in this study. Self-activation circuits include non–phase-separating controls (OP152 and OP177) and phase-separating circuits incorporating intrinsically disordered regions (OP153 and OP203). (C) Colocalization circuits include CT149, which contains 12× lacO operator sites adjacent to the target promoter, and IC41, a matched control lacking lacO sites. All circuits are based on AraC-mediated positive feedback and are expressed in E. coli.

    Article Snippet: BglII-RFP- RFC10 surfix , E1010 , RFP-BglII-F , VR , 880 bp.

    Techniques: Sequencing, Construct, Activation Assay, Control